MiR-490 alleviates sepsis-induced acute lung injury by targeting MRP4 in new-born mice

The aim of this study was to investigate whether the effects miR-490 on acute lung injury (ALI) induced by sepsis in vitro and vivo were through targeting multi-drug resistance-associated protein 4 (MRP4). MiR-490 agomir/NC agomir injected into mice before cecal ligation puncture (CLP). Pulmonary microvascular endothelial cells (PMVECs) transfected with or without agomir/MRP4/empty vector lipopolysaccharide (LPS) stimulation. Histopathology, injure score, Wet/Dry (W/D) tissues assessed. number neutrophils, macrophages total cells, concentration, TNF-α IL-1β level bronchoalveolar lavage fluid (BALF) measured. levels caspase-3, Bcl-2, TNF-α, measured MPVECs. Dual-luciferase reporter assay used analyze relationship between MRP4 miR-490. When compared sham group, CLP mice, alveolar tissue showed significantly hyperemic, collapse, W/D ratio increased, index increased. increased BALF from mice. Overexpression alleviated caused inhibited inflammation caspase-3 but Bcl-2 decreased MPVECs treated LPS control group. also reversed increase IL-1β, cleaved confirmed that target gene MRP4. Besides, overexpression upregulated downregulated transfection. These data suggested could relieve sepsis-induced neonatal via